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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
16s Rrna Sequencing Results, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
Resultant Fragmented Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
Illumina Miseq Sequencing Results, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequence alignment results  (DNASTAR)
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
Sequence Alignment Results, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
Generation Sequencing Results, supplied by Foundation Medicine Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotyping by sequencing of e. oleifera results  (Biotechnology Information)
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
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mrna/ non-coding rna sequencing and results analysis  (Novogene)
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
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sequencing results  (Illumina Inc)
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Phylogenetic analysis based on <t>16S</t> <t>rRNA</t> ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).
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Phylogenetic analysis based on 16S rRNA ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).

Journal: ASM Case Reports

Article Title: Mycobacterium wolinskyi as an emerging cause of pacemaker pocket infection and lead endocarditis: a case report and genomic characterization

doi: 10.1128/asmcr.00146-25

Figure Lengend Snippet: Phylogenetic analysis based on 16S rRNA ( A ), hsp65 ( B ) , rpoB ( C ), and recA ( D ) sequences for several fast-growing Mycobacteria . For the 16S rRNA gene, the corresponding near full-length nucleotide sequence (1,388 bp) was used, while for the hsp65 (372 bp) , rpoB (723 bp), and recA (951 bp) genes, a partial sequence of identical length was used for the phylogenetic analysis, which was performed via the Geneious Tree Builder using the Neighbor-Joining tree build method and the Tamura-Nei Genetic Distance model (Geneious Prime GraphPad software). In cases where a particular strain harbored multiple 16S rRNA copies, these are indicated by (1) and (2).

Article Snippet: Because of discrepant 16S rRNA sequencing results and low ANI percentage compared with the M. wolinskyi reference genome (strain ATCC 700010), a phylogenetic analysis was performed.

Techniques: Sequencing, Software